Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

241499E7

N° de cat : 84192-4-RR

Synonymes

KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Galerie de données de validation

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  WB analysis using 84192-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84192-4-RR (Ki-67 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Spleen using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Spleen using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Spleen tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human renal cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human renal cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded Human renal cancer slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human Breast cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human Breast cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human Breast cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 84192-4-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84192-4-RR (Ki-67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human thyroid cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human thyroid cancer using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1/CD54 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

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  IF Staining of HeLa using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of U2OS using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

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  IF Staining of A431 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of HepG2 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of U2OS using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

• 

  IF Staining of MCF-7 using 84192-4-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Ki-67 antibody (84192-4-RR, Clone: 241499E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red) (Cat No. PF00003).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules MCF-7, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu de cancer de la prostate humain, tissu de cancer du sein humain, tissu de mélanome malin humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du poumon humain, tissu de cancer de la thyroïde humain
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431, cellules A549, cellules HepG2, cellules MCF-7, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

84192-4-RR cible Ki-67 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Poids moléculaire observé: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publications

Species	Application	Title
human	IHC	Cell Biol Int; Hypoxic Cancer Cells-Derived Exosomes Strengthen the Development of Cancer Stem Cell-Like Properties Through Delivering LINC00665 in Thyroid Cancer Cells; Authors - Ming Zhou
human	IF	Int Immunopharmacol; ACNO hydrogel enhances diabetic wound healing by modulating the Bcl-2/Bax/Caspase-3/PARP pathway; Authors - Penghui Ye
human	IHC	Cancer Lett; Apigenin Inhibits Recurrent Bladder Cancer Progression by Targeting VEGF-β; Authors - Zhen-Duo Shi

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