Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241768E2

N° de cat : 84432-1-RR

Synonymes

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 84432-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 84432-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of C2C12 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of NIH/3T3 using 84432-1-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: tissu splénique de souris, tissu splénique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

84432-1-RR cible Ki-67 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351kDa
• Poids moléculaire observé: 350 kDa
• Numéro d’acquisition GenBank: NM_001081117.2
• Symbole du gène: ki67
• Identification du gène (NCBI): 17345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

Publications

Species	Application	Title
mouse	WB,IF	Small; IL-21 Loading CaMnCO3 Vitality Backpacks Boost CAR-T Cell Synergistic Immunotherapy; Authors - Baihui Wang

{{ptg:RelatedPrimaryAntibodies}}