Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

241768E2

N° de cat : 98224-1-RR

Synonymes

Ki 67, Ki67, Mki67, 241768E2, Proliferation related Ki 67 antigen

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Galerie de données de validation

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  FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR

  1x10^6 untreated or Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of C57BL/6 mouse splenocytes using 98224-1-RR

  1x10^6 Con A treated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated C57BL/6 mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse Ki-67 Rabbit Recombinant Antibody (98224-1-RR, Clone: 241768E2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 98224-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98224-1-RR against Mouse Ki-67 were performed. The affinity constant is 1.97 nM.

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Applications testées

• Résultats positifs en FC (Intra): Con A treated C57BL/6 mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98224-1-RR cible Ki-67 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Eg1548
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351kDa
• Numéro d’acquisition GenBank: NM_001081117.2
• Symbole du gène: ki67
• Identification du gène (NCBI): 17345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki-67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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