Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241499B1

N° de cat : CL647-98143

Synonymes

KI67, MKI67, 241499B1, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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Galerie de données de validation

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  FC experiment of human PBMCs using CL647-98143

  1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of human PBMCs using CL647-98143

  1x10^6 PHA-treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human Ki-67 Rabbit RecAb (CL647-98143, Clone: 241499B1) (black, dashed). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en FC (Intra): PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98143 cible Ki-67 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: antigen identified by monoclonal antibody Ki-67
• Masse moléculaire calculée: 359 kDa
• Numéro d’acquisition GenBank: NM_002417
• Symbole du gène: KI67
• Identification du gène (NCBI): 4288
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

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