Anticorps Recombinant de lapin anti-Ki-67

Ki-67 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241768E2

N° de cat : 84432-1-PBS

Synonymes

Mki67, Ki67, Ki 67, Proliferation related Ki 67 antigen, 241768E2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84432-1-RR (Ki67/MKI67 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 84432-1-RR (same clone as 84432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84432-1-RR (Ki-67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C2C12 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 84432-1-RR (same clone as 84432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Ki-67 antibody (84432-1-RR, Clone: 241768E2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84432-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01277-1

  Cytometric bead array standard curve of MP01277-1, MOUSE Ki-67 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84432-2-PBS. Detection antibody: 84432-1-PBS. Standard: Eg1548. Range: 0.625-40 ng/mL.

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Informations sur le produit

84432-1-PBS cible Ki-67 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351kDa
• Poids moléculaire observé: 350 kDa
• Numéro d’acquisition GenBank: NM_001081117.2
• Symbole du gène: ki67
• Identification du gène (NCBI): 17345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

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