Anticorps Polyclonal de lapin anti-Kindlin 1

• Phare
• Validé par KD/KO

Kindlin 1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22215-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 22215-1-AP (same clone as 22215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22215-1-AP (Kindlin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 22215-1-AP (same clone as 22215-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22215-1-AP (FERMT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (Kindlin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22215-1-AP (FERMT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 22215-1-AP (same clone as 22215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22215-1-AP (FERMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 22215-1-AP (same clone as 22215-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 22215-1-AP (FERMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22215-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22215-1-PBS cible Kindlin 1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kindlin 1 Protéine recombinante Ag17543
• Nom complet: fermitin family homolog 1 (Drosophila)
• Masse moléculaire calculée: 677 aa, 77 kDa
• Poids moléculaire observé: 70-77 kDa
• Numéro d’acquisition GenBank: BC035882
• Symbole du gène: Kindlin 1
• Identification du gène (NCBI): 55612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Kindlin-1 is a FERM domain containing adaptor protein that is found predominantly at cell-extracellular matrix adhesions where it binds to β-integrin subunits and is required for integrin activation. Loss of function mutations in the FERMT1 gene which encodes Kindlin-1 leads to the development of Kindler Syndrome (KS) an autosomal recessive skin disorder characterized by skin blistering, photosensitivity, and predisposition to aggressive squamous cell carcinoma (SCC).

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