Anticorps Polyclonal de lapin anti-Kindlin 3

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18131-1-PBS

Synonymes

FERMT3, KIND3, Kindlin-3, MIG 2, MIG2 like protein

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Galerie de données de validation

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  WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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  WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 18131-1-AP (same clone as 18131-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 18131-1-AP (FERMT3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 18131-1-AP (same clone as 18131-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 18131-1-AP (same clone as 18131-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18131-1-PBS cible Kindlin 3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kindlin 3 Protéine recombinante Ag12713
• Nom complet: fermitin family homolog 3 (Drosophila)
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 72-76 kDa
• Numéro d’acquisition GenBank: BC004347
• Symbole du gène: Kindlin 3/FERMT3
• Identification du gène (NCBI): 83706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.

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