Anticorps Recombinant de lapin anti-L-Plastin

L-Plastin Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251063A3

N° de cat : 86299-3-PBS

Synonymes

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

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Galerie de données de validation

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  WB analysis using 86299-3-RR (same clone as 86299-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86299-3-RR (L-Plastin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86299-3-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 86299-3-RR (same clone as 86299-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using L-Plastin antibody (86299-3-RR, Clone: 251063A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86299-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86299-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86299-3-RR against Human L-Plastin were performed. The affinity constant is 5.27 nM.

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Informations sur le produit

86299-3-PBS cible L-Plastin dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: L-Plastin Protéine recombinante Ag3571
• Nom complet: lymphocyte cytosolic protein 1 (L-plastin)
• Masse moléculaire calculée: 627 aa, 64 kDa
• Poids moléculaire observé: 64-70 kDa
• Numéro d’acquisition GenBank: BC010271
• Symbole du gène: L-Plastin
• Identification du gène (NCBI): 3936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).

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