Anticorps Polyclonal de lapin anti-CACNA1C

CACNA1C Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21774-1-PBS

Synonymes

CACH2, CACN2, CACNL1A1, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, CCHL1A1

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Galerie de données de validation

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  WB analysis using 21774-1-AP (same clone as 21774-1-PBS)

  Mouse heart lysate was subjected to SDS PAGE, followed by western blot with 21774-1-AP (CACNA1C antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21774-1-AP (L-VOCC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver using 21774-1-AP (L-VOCC antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21774-1-AP (L-VOCC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 21774-1-AP (same clone as 21774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using L-VOCC antibody (21774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 21774-1-AP (same clone as 21774-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human L-VOCC (21774-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 21774-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21774-1-PBS cible CACNA1C dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CACNA1C Protéine recombinante Ag16455
• Nom complet: calcium channel, voltage-dependent, L type, alpha 1C subunit
• Masse moléculaire calculée: 249 kDa
• Poids moléculaire observé: 249 kDa
• Numéro d’acquisition GenBank: BC146846
• Symbole du gène: CACNA1C
• Identification du gène (NCBI): 775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Calcium voltage-gated channel subunit alpha1 C (CACNA1C, also known as CACH2 and Cav1.2) couples transient activation of inward calcium current to transcriptional regulation and plays an important role in dendritic development, neuronal survival, synaptic plasticity, memory formation, learning, and behavior (PMID: 21248242;  16251435; 20169575; 19047462; 18174367). Genetic variation in CACNA1C has also been associated with depression, schizophrenia, and autism spectrum disorders, as well as changes in brain function and structure in control subjects who have no diagnosable psychiatric illness (PMID: 22705413).

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