Anticorps Polyclonal de lapin anti-L1CAM/CD171

L1CAM/CD171 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 32885-1-AP

Synonymes

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

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Galerie de données de validation

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  WB analysis using 32885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32885-1-AP (L1CAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 32885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Neuro-2a using 32885-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using L1CAM antibody (32885-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32885-1-AP cible L1CAM/CD171 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L1CAM/CD171 Protéine recombinante Eg3022
• Nom complet: L1 cell adhesion molecule
• Masse moléculaire calculée: 141 kDa
• Poids moléculaire observé: 200-220 kDa, 140 kDa
• Numéro d’acquisition GenBank: N/A
• Symbole du gène: L1cam
• Identification du gène (NCBI): 16728
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733).

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