Anticorps Recombinant de lapin anti-L1CAM/CD171

L1CAM/CD171 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240978A7

N° de cat : 98130-1-RR

Synonymes

L1CAM, 240978A7, CAML1, CD171, HSAS

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Galerie de données de validation

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  FC experiment of SK-N-SH using 98130-1-RR

  1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone:240978A7) or 0.25 ug Isotype Control, and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

• 

  FC experiment of SK-N-SH using 98130-1-RR

  1x10^6 SK-N-SH cells were surface stained with 0.25 ug Anti-Human L1CAM/CD171 Rabbit Recombinant Antibody (98130-1-RR, Clone: 240978A7) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98130-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98130-1-RR against Human L1CAM/CD171 were performed. The affinity constant is 0.243 nM.

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Applications testées

• Résultats positifs en cytométrie: SK-N-SH cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98130-1-RR cible L1CAM/CD171 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: L1CAM/CD171 Protéine recombinante Eg0209
• Nom complet: L1 cell adhesion molecule
• Masse moléculaire calculée: 1257 aa, 140 kDa
• Numéro d’acquisition GenBank: BC126229
• Symbole du gène: L1CAM
• Identification du gène (NCBI): 3897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

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