Anticorps Polyclonal de lapin anti-L2HGDH

• Phare
• Validé par KD/KO

L2HGDH Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15707-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 15707-1-AP (same clone as 15707-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH Antibody) at dilution of 1:800 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse small intestine using 15707-1-AP (same clone as 15707-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 15707-1-AP (same clone as 15707-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15707-1-AP (same clone as 15707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15707-1-AP (L2HGDH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 15707-1-AP (same clone as 15707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15707-1-AP (L2HGDH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15707-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15707-1-PBS cible L2HGDH dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: L2HGDH Protéine recombinante Ag8382
• Nom complet: L-2-hydroxyglutarate dehydrogenase
• Masse moléculaire calculée: 463aa,50 kDa; 441aa,49 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC006117
• Symbole du gène: L2HGDH
• Identification du gène (NCBI): 79944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

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