Anticorps Monoclonal anti-L2HGDH

L2HGDH Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3D11C2

N° de cat : 66269-1-PBS

Synonymes

C14orf160, DURANIN, L2HGDH

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Galerie de données de validation

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  WB analysis of pig brain using 66269-1-Ig (same clone as 66269-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66269-1-Ig (same clone as 66269-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66269-1-Ig (L2HGDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66269-1-Ig (L2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66269-1-Ig (same clone as 66269-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using L2HGDH antibody (66269-1-Ig, Clone: 3D11C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66269-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66269-1-PBS cible L2HGDH dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: L2HGDH Protéine recombinante Ag8382
• Nom complet: L-2-hydroxyglutarate dehydrogenase
• Masse moléculaire calculée: 463aa,50 kDa; 441aa,49 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC006117
• Symbole du gène: L2HGDH
• Identification du gène (NCBI): 79944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

L2HGDH(L-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as duranin, C14orf160 and belongs to the L2HGDH family. The putative L2HGDH is predicted to be targeted to the mitochondria where its mitochondrial targeting sequence is presumably removed(PMID:16005139). Defects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA). It has 2 isoforms produced by alternative splicing with the molecular weight of 50 kDa and 48 kDa. L2HGDH also can be detected as ~45kD due to the 51aa transit peptide cleaved.

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