Anticorps Monoclonal anti-LACTB

• Phare
• Validé par KD/KO

LACTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

3C3C3

N° de cat : 66785-1-Ig

Synonymes

3C3C3, EC:3.4.-.-, G24, lactamase, beta, MRPL56

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Galerie de données de validation

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  WB analysis using 66785-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-Ig (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66785-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 66785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 66785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1600 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HepG2 using 66785-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

66785-1-Ig cible LACTB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LACTB Protéine recombinante Ag12233
• Nom complet: lactamase, beta
• Masse moléculaire calculée: 547 aa, 61 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC067288
• Symbole du gène: LACTB
• Identification du gène (NCBI): 114294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma ; KD Validated
human	WB	Cancer Manag Res; MicroRNA-1276 Promotes Colon Cancer Cell Proliferation by Negatively Regulating LACTB.; Authors - Chunxiao Wang
mouse,human	WB	Mol Cell; OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB; Authors - Wenhao Ma
human	WB,IP	STAR Protoc; Protocol for identifying OXCT1-mediated LACTB succinylation sites in vitro; Authors - Wenhao Ma

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