Anticorps Polyclonal de lapin anti-LAG-3/CD223

LAG-3/CD223 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 16616-1-PBS

Synonymes

CD223, LAG3, FDC, LAG 3, LAG-3

---

Galerie de données de validation

• 

  WB analysis using 16616-1-AP (same clone as 16616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

  IP result of anti-LAG-3 (IP:16616-1-AP, 4ug; Detection:16616-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

16616-1-PBS cible LAG-3/CD223 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAG-3/CD223 Protéine recombinante Ag9909
• Nom complet: lymphocyte-activation gene 3
• Masse moléculaire calculée: 525 aa, 57 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC052589
• Symbole du gène: LAG-3
• Identification du gène (NCBI): 3902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

{{ptg:RelatedPrimaryAntibodies}}