Anticorps Recombinant de lapin anti-LAG-3/CD223

LAG-3/CD223 Recombinant Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241007B1

N° de cat : 83941-7-PBS

Synonymes

CD223, LAG-3, LAG 3, Lag3, Ly66

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Galerie de données de validation

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  IHC staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83941-7-RR (same clone as 83941-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83941-7-RR (LAG-3/CD223 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83941-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83941-7-PBS

  Biolayer interferometry (BLl) kinetic assay of 83941-7-PBS against Mouse LAG-3/CD223 was performed. The affinity constant is 61.3 nM.

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Informations sur le produit

83941-7-PBS cible LAG-3/CD223 dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAG-3/CD223 Protéine recombinante Eg1220
• Nom complet: lymphocyte-activation gene 3
• Masse moléculaire calculée: 57 kDa
• Numéro d’acquisition GenBank: NM_008479.2
• Symbole du gène: CD223
• Identification du gène (NCBI): 16768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

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