Anticorps Recombinant de lapin anti-LAG-3/CD223

LAG-3/CD223 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

243086D4

N° de cat : FITC-98435-2

Synonymes

CD223, LAG3, FDC, LAG 3, LAG-3

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-98435-2

  1x10^6 untreated or anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with CoraLite®594 Anti-Human CD8 and 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98435-2

  1x10^6 anti-CD3/CD28 and IL-2 treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human LAG-3/CD223 Rabbit RecAb (FITC-98435-2, Clone: 243086D4) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 and IL-2 treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98435-2 cible LAG-3/CD223 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAG-3/CD223 Protéine recombinante Eg31550
• Nom complet: lymphocyte-activation gene 3
• Masse moléculaire calculée: 57 kDa
• Numéro d’acquisition GenBank: NM_002286
• Symbole du gène: LAG-3
• Identification du gène (NCBI): 3902
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

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