Anticorps Recombinant de lapin anti-LAG-3/CD223

LAG-3/CD223 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1F14

N° de cat : 80867-1-RR

Synonymes

LAG-3, CD223, 1F14, LAG3, LAG 3

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Galerie de données de validation

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  WB analysis of CTLL-2 using 80867-1-RR

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 80867-1-RR (LAG-3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80867-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80867-1-RR against Human LAG-3/CD223 were performed. The affinity constant is 8.47 nM.

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Applications testées

• Résultats positifs en WB: CTLL-2 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

80867-1-RR cible LAG-3/CD223 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: lymphocyte-activation gene 3
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_002286
• Symbole du gène: LAG-3
• Identification du gène (NCBI): 3902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

Publications

Species	Application	Title
human	WB	Cancer Sci; Screening of novel serum biomarkers for gastric cancer in coastal populations using a protein microarray; Authors - Yongdong Yi
mouse	IF	Cancer Lett; The deubiquitinase USP2a promotes tumor immunosuppression by stabilizing immune checkpoint B7-H4 in lung adenocarcinoma harboring EGFR-activating mutants; Authors - Youwei Lu
mouse	WB,IF	Neural Regen Res; Neuroprotective effects of lixisenatide against propagation of α-synuclein pathology in Parkinson's disease; Authors - Shangqi Sun
human	WB	Int Immunopharmacol; Targeting SPP1+ macrophages via the SPP1-CD44 axis reveals a key mechanism of immune suppression and tumor progression in ovarian cancer; Authors - Lisha Hou

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