Anticorps Monoclonal anti-LAIR1

LAIR1 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A5D1

N° de cat : 67220-1-Ig

Synonymes

CD305, hLAIR1, LAIR 1, LAIR1

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Galerie de données de validation

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  WB analysis of THP-1 using 67220-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 67220-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of TF-1 using 67220-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 67220-1-Ig (LAIR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 67220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67220-1-Ig (LAIR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 67220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 67220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAIR1 antibody (67220-1-Ig, Clone: 1A5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HL-60, cellules TF-1
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67220-1-Ig cible LAIR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LAIR1 Protéine recombinante Ag14459
• Nom complet: leukocyte-associated immunoglobulin-like receptor 1
• Masse moléculaire calculée: 287 aa, 31 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC027899
• Symbole du gène: LAIR1
• Identification du gène (NCBI): 3903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) is a transmembrane glycoprotein with a single immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs (PMID: 9285412). LAIR1 is expressed on the majority of human PBMCs, including NK, T, B, monocytes, and dendritic cells, as well as the majority of thymocytes (PMID: 10229813). It functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of NK cells, B cells and T cells. LAIR1 is a collagen receptor (PMID: 16754721). Tumor-expressed collagens can modulate immune cell function through the inhibitory collagen receptor LAIR1 (PMID: 21955987).

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Med; Development and validation of a leukocyte-associated immunoglobulin-like receptor-1 prognostic signature for lower-grade gliomas.; Authors - Zhansheng Fang