Anticorps Polyclonal de lapin anti-LAMA1

LAMA1 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 28697-1-AP

Synonymes

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin subunit alpha-1, Laminin subunit alpha 1

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Galerie de données de validation

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  WB analysis using 28697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of rat colon using 28697-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules U2OS
• Résultats positifs en IF-P: tissu de côlon de rat,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28697-1-AP cible LAMA1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMA1 Protéine recombinante Ag29329
• Nom complet: laminin, alpha 1
• Masse moléculaire calculée: 337 kDa
• Poids moléculaire observé: 337 kDa
• Numéro d’acquisition GenBank: NM_005559
• Symbole du gène: LAMA1
• Identification du gène (NCBI): 284217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)

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