Anticorps Recombinant de lapin anti-LAMA3

LAMA3 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

230443C1

N° de cat : 83210-2-PBS

Synonymes

230443C1, BM600, E170, lama3a, Laminin subunit alpha 3

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Galerie de données de validation

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  WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00186-2

  Sandwich ELISA standard curve of MP00186-2, Human LAMA3 Recombinant Matched Antibody Pair - PBS only. 83210-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01122. 83210-2-PBS was HRP conjugated as the detection antibody. Range: 0.131-20 ng/mL.

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  Sample test of MP00186-2

  The mean LAMA3 concentration was determined to be 27.50 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load, 51.60 ng/mL in HepG2 cell extract based on a 5.00 mg/mL extract load and 21.80 ng/mL in A431 cell extract based on a 2.60 mg/mL extract load.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00186-2

  Cytometric bead array standard curve of MP00186-2, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-1-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-10 ng/mL.

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Informations sur le produit

83210-2-PBS cible LAMA3 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: laminin, alpha 3
• Masse moléculaire calculée: 367 kDa
• Poids moléculaire observé: 260 kDa
• Numéro d’acquisition GenBank: NM_000227
• Symbole du gène: LAMA3
• Identification du gène (NCBI): 3909
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)

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