Anticorps Polyclonal de lapin anti-LAMB2

LAMB2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 30943-1-AP

Synonymes

S LAM beta, LAMS, Laminin 3 subunit beta, Laminin 15 subunit beta, Laminin 14 subunit beta

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Galerie de données de validation

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30943-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 30943-1-AP (LAMB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 30943-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30943-1-AP (LAMB2 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30943-1-AP cible LAMB2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMB2 Protéine recombinante Ag33468
• Nom complet: laminin, beta 2 (laminin S)
• Masse moléculaire calculée: 196 kDa
• Poids moléculaire observé: 195-200 kDa
• Numéro d’acquisition GenBank: NM_002292.3
• Symbole du gène: LAMB2
• Identification du gène (NCBI): 3913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMB2 (laminin subunit beta 2), also known as LAMS. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. This gene encodes the beta chain isoform laminin, beta 2. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. The calculated molecular weight of LAMB2 is 200 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (06-16-2025)

works well, clean bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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