Anticorps Recombinant de lapin anti-LAMB3

LAMB3 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242442A8

N° de cat : 85055-4-PBS

Synonymes

Laminin B1k chain, 242442A8, Epiligrin subunit bata, Kalinin B1 chain, Kalinin subunit beta

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Galerie de données de validation

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  WB analysis using 85055-4-RR (same clone as 85055-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85055-4-RR (LAMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 85055-4-RR (same clone as 85055-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85055-4-RR (LAMB3 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85055-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85055-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85055-4-RR against Human LAMB3 were performed. The affinity constant is 0.244 nM.

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Informations sur le produit

85055-4-PBS cible LAMB3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAMB3 Protéine recombinante Ag25192
• Nom complet: laminin, beta 3
• Masse moléculaire calculée: 1172 aa, 130 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC075838
• Symbole du gène: LAMB3
• Identification du gène (NCBI): 3914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAMB3 (β3 subunit of laminin) is an important extracellular matrix protein, which plays an important role in cell adhesion, migration, signal transduction and the formation and stability of basement membrane. LAMB3 plays a key role in the basement membrane of skin and mucosa, maintaining the adhesion between epidermis and dermis. Its abnormal function will lead to skin bullous disease (such as severe borderline epidermolaxis, sJEB), which is characterized by blisters and ulcers on skin and mucosa. LAMB3 is highly expressed in many cancers, including pancreatic ductal adenocarcinoma (PDAC), lung cancer, head and neck squamous cell carcinoma, etc., which is closely related to the proliferation, invasion and metastasis of tumor cells.

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