Anticorps Monoclonal anti-LAMC1

• Phare
• Validé par KD/KO

LAMC1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1G2G4

N° de cat : 67706-1-Ig

Synonymes

Laminin subunit gamma 1, 1G2G4, LAMB2, LAMC 1, Laminin subunit gamma-1

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Galerie de données de validation

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  WB analysis using 67706-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67706-1-Ig

  WB result of LAMC1 antibody (67706-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAMC1 transfected HeLa cells.

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  WB analysis of human placenta using 67706-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67706-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat rectum using 67706-1-Ig

  rat rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig rectum using 67706-1-Ig

  pig rectum tissue were subjected to SDS PAGE followed by western blot with 67706-1-Ig (LAMC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67706-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LAMC1 antibody (67706-1-Ig, Clone: 1G2G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules JAR, cellules LNCaP, tissu placentaire humain, tissu rectal de porc, tissu rectal de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

67706-1-Ig cible LAMC1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LAMC1 Protéine recombinante Ag14674
• Nom complet: laminin, gamma 1 (formerly LAMB2)
• Masse moléculaire calculée: 178 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: BC015586
• Symbole du gène: LAMC1
• Identification du gène (NCBI): 3915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Neuroscience; Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety.; Authors - Dan Liu
human	WB	Rheumatology (Oxford); The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies; Authors - Yongxin Mo
rat,mouse	WB,IF	Exp Neurol; LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage; Authors - Qiaowei Wu
human	WB	J Transl Med; HIF-1α-mediated LAMC1 overexpression is an unfavorable predictor of prognosis for glioma patients: evidence from pan-cancer analysis and validation experiments; Authors - Jianrong Bai

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