Anticorps Polyclonal de lapin anti-CD107b / LAMP2

CD107b / LAMP2 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

40

N° de cat : 27823-1-AP

Synonymes

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

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Galerie de données de validation

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  WB analysis using 27823-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27823-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 27823-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 27823-1-AP (LAMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27823-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27823-1-AP (LAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, tissu hépatique de porc, tissu placentaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 15 publications below

Informations sur le produit

27823-1-AP cible CD107b / LAMP2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD107b / LAMP2 Protéine recombinante Ag27183
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC002965
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

Publications

Species	Application	Title
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
	WB	Autophagy; USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.; Authors - Xibao Zhao
pig	IF	Autophagy; Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture; Authors - Tao Wang
human	WB	Oxid Med Cell Longev; Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration.; Authors - Liang Kang
pig	WB	J Anim Sci Biotechnol; Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.; Authors - Chunhua Xing
	IF	J Neurosci; Effect of Aβ oligomers on neuronal APP triggers a vicious cycle leading to the propagation of synaptic plasticity alterations to healthy neurons.; Authors - Marta Rolland