Anticorps Recombinant de lapin anti-CD107b / LAMP2

CD107b / LAMP2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250948D7

N° de cat : 86269-3-PBS

Synonymes

CD107b, LAMP2, CD107 antigen-like family member B, LAMP 2, LAMP-2

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Galerie de données de validation

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  WB analysis using 86269-3-RR (same clone as 86269-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86269-3-PBS in a different storage buffer formulation.

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  WB analysis using 86269-3-RR (same clone as 86269-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86269-3-RR (LAMP2/ CD107b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86269-3-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 86269-3-RR (same clone as 86269-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86269-3-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocellular cancer using 86269-3-RR (same clone as 86269-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 86269-3-RR (CD107b / LAMP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86269-3-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 86269-3-RR (same clone as 86269-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LAMP2/ CD107b antibody (86269-3-RR, Clone: 250948D7 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86269-3-PBS in a different storage buffer formulation.

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Informations sur le produit

86269-3-PBS cible CD107b / LAMP2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD107b / LAMP2 Protéine recombinante Eg4998
• Nom complet: lysosomal-associated membrane protein 2
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: NM_002294.3
• Symbole du gène: LAMP2
• Identification du gène (NCBI): 3920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

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