Anticorps Recombinant de lapin anti-LAMP5

LAMP5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230102F12

N° de cat : 82992-2-RR

Synonymes

C20orf103, LAMP-5, Brain-associated LAMP-like protein, Brain and dendritic cell-associated LAMP, BAD-LAMP

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Galerie de données de validation

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  WB analysis using 82992-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82992-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82992-2-RR (LAMP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 82992-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of Neuro-2a using 82992-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using LAMP5 antibody (82992-2-RR, Clone: 230102F12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of SH-SY5Y using 82992-2-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug LAMP5 Recombinant antibody (82992-2-RR, Clone:230102F12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82992-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82992-2-RR against Human LAMP5 were performed. The affinity constant is 0.247 nM.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules U-937
• Résultats positifs en IF/ICC: cellules Neuro-2a, cellules SH-SY5Y
• Résultats positifs en FC (Intra): cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82992-2-RR cible LAMP5 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAMP5 Protéine recombinante Ag33894
• Nom complet: chromosome 20 open reading frame 103
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC050727
• Symbole du gène: LAMP5
• Identification du gène (NCBI): 24141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMP5 (Lysosome-associated membrane glycoprotein 5) is also named as BAD-LAMP and C20orf103. BAD-LAMP is a novel biomarker of nonactivated human plasmacytoid dendritic cells (PMID: 21642595). LAMP5 may be one of the key genes in the development of Multiple myeloma (MM) as well as its recurrence  (PMID: 37033323). LAMP5 is a mammalian ortholog of the Caenorhabditis elegans protein, UNC-46, which functions as a sorting factor to localize the vesicular GABA transporter UNC-47 to synaptic vesicles (PMID: 30867010). It was localized exclusively in inhibitory synaptic terminals (PMID: 30867010).

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