Anticorps Polyclonal de lapin anti-LAMTOR1

LAMTOR1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 29611-1-AP

Synonymes

C11orf59, p27RF-Rho, p27RF Rho, p27Kip1-releasing factor from RhoA, Lipid raft adaptor protein p18

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Galerie de données de validation

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  WB analysis using 29611-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29611-1-AP (LAMTOR1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-87 MG using 29611-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using LAMTOR1 antibody (29611-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en IF/ICC: cellules MG U-87,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29611-1-AP cible LAMTOR1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAMTOR1 Protéine recombinante Ag31215
• Nom complet: chromosome 11 open reading frame 59
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC001706
• Symbole du gène: LAMTOR1
• Identification du gène (NCBI): 55004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAMTOR1 (Late Endosome/Lysosome-Associated Membrane Protein 1) is a membrane protein specifically localized to the surface of late endosomes/lysosomes and is involved in lysosomal transport and maturation. LAMTOR1 is a key component of the lysosomal articulating protein complex Ragulator, which is required for the localization of the Ragulator complex to lysosomes. LAMTOR1 is a key component of the lysosomal bridging protein complex Ragulator and is required for the localization of the Ragulator complex to the lysosome. LAMTOR1 is involved in autophagy, cell growth, immune regulation, cancer development, and regulatory pathways of glucose metabolism. Recent studies have shown that in calorie restriction, lithocholic acid activates AMPK through the glucose-sensing pathway ( the lysosomal pathway ) and the body senses the metabolic signals induced by calorie restriction and exerts its role in slowing down the effects of aging and prolonging the lifespan, whereas deletion of LAMTOR1 results in the inability of AMPK to be activated in order to play its role.

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