Anticorps Monoclonal anti-LANCL1

• Phare
• Validé par KD/KO

LANCL1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H6E8

N° de cat : 68160-1-PBS

Synonymes

2H6E8, EC:2.5.1.18, Glutathione S-transferase LANCL1, GPR69A, LanC like protein 1

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Galerie de données de validation

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  WB analysis using 68160-1-Ig (same clone as 68160-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68160-1-Ig (same clone as 68160-1-PBS)

  WB result of LANCL1 antibody (68160-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LANCL1 transfected HeLa cells. This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 68160-1-Ig (same clone as 68160-1-PBS)

  IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug. This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 68160-1-Ig (same clone as 68160-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68160-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68160-1-PBS cible LANCL1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LANCL1 Protéine recombinante Ag32665
• Nom complet: LanC lantibiotic synthetase component C-like 1 (bacterial)
• Masse moléculaire calculée: 399 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC028685
• Symbole du gène: LANCL1
• Identification du gène (NCBI): 10314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

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