Anticorps Polyclonal de lapin anti-LAP3

LAP3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14612-1-AP

Synonymes

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

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Galerie de données de validation

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  WB analysis of HeLa using 14612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14612-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14612-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14612-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules SH-SY5Y, tissu cardiaque de souris, tissu pulmonaire de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

14612-1-AP cible LAP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LAP3 Protéine recombinante Ag6205
• Nom complet: leucine aminopeptidase 3
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC065564
• Symbole du gène: LAP3
• Identification du gène (NCBI): 51056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684).

Publications

Species	Application	Title
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Reprod Toxicol; 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.; Authors - Shaoping Huang
human	WB	Cancer Genomics Proteomics; Proteomic Analysis of Hepatocellular Carcinoma Tissues With Encapsulation Shows Up-regulation of Leucine Aminopeptidase 3 and Phosphoenolpyruvate Carboxykinase 2.; Authors - Keisuke Kuhara