Anticorps Monoclonal anti-LAP3

LAP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B11B8

N° de cat : 66417-1-PBS

Synonymes

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of C2C12 using 66417-1-Ig (same clone as 66417-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66417-1-Ig (same clone as 66417-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66417-1-PBS cible LAP3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LAP3 Protéine recombinante Ag6300
• Nom complet: leucine aminopeptidase 3
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC065564
• Symbole du gène: LAP3
• Identification du gène (NCBI): 51056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.

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