Anticorps Polyclonal de lapin anti-LARG

• Phare
• Validé par KD/KO

LARG Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22441-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 22441-1-AP (same clone as 22441-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22441-1-AP (same clone as 22441-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

  Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22441-1-PBS cible LARG dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LARG Protéine recombinante Ag18211
• Nom complet: Rho guanine nucleotide exchange factor (GEF) 12
• Masse moléculaire calculée: 1544 aa, 173 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC063117
• Symbole du gène: LARG
• Identification du gène (NCBI): 23365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

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