Anticorps Recombinant de lapin anti-LARP7

LARP7 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242561F5

N° de cat : 84950-3-PBS

Synonymes

242561F5, HDCMA18P, hLARP7, La related protein 7, La-related protein 7

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Galerie de données de validation

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  WB analysis using 84950-3-RR (same clone as 84950-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84950-3-RR (LARP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84950-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84950-3-RR (same clone as 84950-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84950-3-RR (LARP7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84950-3-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 84950-3-RR (same clone as 84950-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84950-3-RR (LARP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84950-3-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84950-3-RR (same clone as 84950-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using LARP7 antibody (84950-3-RR, Clone: 242561F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84950-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01697-1

  Cytometric bead array standard curve of MP01697-1, LARP7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84950-3-PBS. Detection antibody: 84950-2-PBS. Standard: Ag10850. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01697-2

  Cytometric bead array standard curve of MP01697-2, LARP7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84950-3-PBS. Detection antibody: 84950-1-PBS. Standard: Ag10850. Range: 1.563-100 ng/mL.

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Informations sur le produit

84950-3-PBS cible LARP7 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LARP7 Protéine recombinante Ag10850
• Nom complet: La ribonucleoprotein domain family, member 7
• Masse moléculaire calculée: 582 aa, 67 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC006981
• Symbole du gène: LARP7
• Identification du gène (NCBI): 51574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.[PMID:19416841].

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