Anticorps Polyclonal de lapin anti-LARS2

• Phare
• Validé par KD/KO

LARS2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17097-1-AP

Synonymes

KIAA0028, LARS2, Leucine tRNA ligase, LEURS

---

Galerie de données de validation

• 

  WB analysis using 17097-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17097-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17097-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17097-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17097-1-AP (LARS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17097-1-AP

  IP Result of anti-LARS2 (IP:17097-1-AP, 4ug; Detection:17097-1-AP 1:500) with Jurkat cells lysate 2000ug.

• 

  IHC staining of human colon cancer using 17097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17097-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17097-1-AP (LARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 17097-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17097-1-AP (LARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 4 publications below

Informations sur le produit

17097-1-AP cible LARS2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LARS2 Protéine recombinante Ag10576
• Nom complet: leucyl-tRNA synthetase 2, mitochondrial
• Masse moléculaire calculée: 903 aa, 102 kDa
• Poids moléculaire observé: 102 kDa
• Numéro d’acquisition GenBank: BC025989
• Symbole du gène: LARS2
• Identification du gène (NCBI): 23395
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LARS2 (Probable leucine--tRNA ligase, mitochondrial) is also named as mitochondrial LEURS. The deduced 903-amino acid protein has an N-terminal mitochondrial targeting signal that is cleaved after residue 39. LARS2 has characteristics of a class I aminoacyl-tRNA synthetase, including a classical Rossmann fold. It belongs to the class-I aminoacyl-tRNA synthetase family.

Publications

Species	Application	Title
mouse	IF	Circulation; Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells.; Authors - David T Paik
human	IF, WB	Mol Cell; Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.; Authors - Sung-Hoon Kim
zebrafish	WB	Nucleic Acids Res; Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.; Authors - Qinghai Zhang
human	IF	EMBO Mol Med; The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells.; Authors - Elena Perli
human,mouse	WB	Oxid Med Cell Longev; LARS2 Regulates Apoptosis via ROS-Mediated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in Ovarian Granulosa Cells.; Authors - Shujun Feng ; KD Validated
human	WB	J Biol Chem; Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.; Authors - Shasha Gong