Anticorps Monoclonal anti-LASP1

• Phare
• Validé par KD/KO

LASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1G4B6

N° de cat : 68080-1-PBS

Synonymes

1G4B6, LASP-1, LIM and SH3 protein 1, MLN50

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Galerie de données de validation

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  WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 68080-1-Ig (same clone as 68080-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68080-1-Ig (same clone as 68080-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68080-1-PBS cible LASP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LASP1 Protéine recombinante Ag18101
• Nom complet: LIM and SH3 protein 1
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC012460
• Symbole du gène: LASP1
• Identification du gène (NCBI): 3927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

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