Anticorps Recombinant de lapin anti-LASS2

LASS2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241898E11

N° de cat : 84514-4-RR

Synonymes

241898E11, Ceramide synthase 2, CerS2, EC:2.3.1.24, EC:2.3.1.297

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Galerie de données de validation

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  WB analysis using 84514-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84514-4-RR (LASS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of Human Hepatocellular cancer using 84514-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84514-4-RR (LASS2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Hepatocellular cancer using 84514-4-RR

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 84514-4-RR (LASS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84514-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84514-4-RR against Human LASS2 were performed. The affinity constant is 0.549 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IHC: Human Hepatocellular cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84514-4-RR cible LASS2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LASS2 Protéine recombinante Ag14151
• Nom complet: LAG1 homolog, ceramide synthase 2
• Masse moléculaire calculée: 380 aa, 45 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC010032
• Symbole du gène: LASS2
• Identification du gène (NCBI): 29956
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LASS2, also known as TMSG1, is a novel suppressor of human cancer metastasis. As one member of LASS family, including LASS1-6, LASS2 mRNA is at the highest level of all LASS members, and has the broadest tissue distribution, particularly abundant in the liver, kidney and brain in mice. The biological roles of LASS2 include protection from aging , hepatic INS resistance, and hepatocellular carcinoma (HCC) progression. LASS2 has been correlated with the degree of invasion and recurrence of carcinomas of the prostate, liver, breast and bladder.

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