Anticorps Recombinant de lapin anti-LAT

• Phare
• Validé par KD/KO

LAT Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2P13

N° de cat : 82901-1-PBS

Synonymes

p36-38, p36 38, Linker for activation of T-cells family member 1, LAT1, 36 kDa phosphotyrosine adapter protein

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Galerie de données de validation

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  WB analysis using 82901-1-RR (same clone as 82901-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82901-1-RR (LAT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  WB result of LAT antibody (82901-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAT transfected Jurkat cells. This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LAT antibody (82901-1-RR, Clone: 2P13 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 82901-1-RR (same clone as 82901-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug LAT Recombinant antibody (82901-1-RR, Clone:2P13) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82901-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82901-1-PBS cible LAT dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: linker for activation of T cells
• Masse moléculaire calculée: 262 aa, 28 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC011563
• Symbole du gène: LAT
• Identification du gène (NCBI): 27040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.

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