Anticorps Polyclonal de lapin anti-LATS1

• Phare
• Validé par KD/KO

LATS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 17049-1-PBS

Synonymes

WARTS, Serine/threonine-protein kinase LATS1, LATS 1, h-warts, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis of HepG2 using 17049-1-AP (same clone as 17049-1-PBS)

  WB result of LATS1 antibody (17049-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  WB analysis using 17049-1-AP (same clone as 17049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 17049-1-AP (same clone as 17049-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 17049-1-AP (same clone as 17049-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17049-1-AP (LATS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17049-1-AP (LATS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17049-1-AP (same clone as 17049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17049-1-AP (LATS1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17049-1-AP (same clone as 17049-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LATS1 antibody (17049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17049-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17049-1-PBS cible LATS1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LATS1 Protéine recombinante Ag10709
• Nom complet: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Masse moléculaire calculée: 15 kDa, 127 kDa
• Poids moléculaire observé: 140-150 kDa, 120 kDa
• Numéro d’acquisition GenBank: BC015665
• Symbole du gène: LATS1
• Identification du gène (NCBI): 9113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

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