Anticorps Recombinant de lapin anti-LAYN

LAYN Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250175B7

N° de cat : 85900-1-PBS

Synonymes

Layilin, UNQ208/PRO234

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Galerie de données de validation

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  WB analysis using 85900-1-RR (same clone as 85900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85900-1-RR (LAYN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85900-1-RR (same clone as 85900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85900-1-RR (LAYN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 85900-1-RR (same clone as 85900-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAYN antibody (85900-1-RR, Clone: 250175B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85900-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85900-1-RR against Human LAYN were performed. The affinity constant is 7.21 nM.

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Informations sur le produit

85900-1-PBS cible LAYN dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LAYN Protéine recombinante Ag14508
• Nom complet: layilin
• Masse moléculaire calculée: 382 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC025407
• Symbole du gène: LAYN
• Identification du gène (NCBI): 143903
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LAYN acts as a receptor for hyaluronic acid (HA) and is involved in various cellular processes, including cell adhesion, movement, and signal transduction. In the context of cancer, LAYN has been identified as a prognostic biomarker. High expression of LAYN is associated with poor prognosis and increased immune cell infiltration in various cancers, such as colorectal cancer and gastric cancer. It may also play a role in T-cell exhaustion and the regulation of tumor-associated macrophages (PMID: 30761122).

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