Anticorps Polyclonal de lapin anti-LBP

LBP Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11836-1-AP

Synonymes

LBP

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Galerie de données de validation

• 

  WB analysis of mouse liver using 11836-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11836-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11836-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11836-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11836-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11836-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 11836-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11836-1-AP (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11836-1-AP

  IP Result of anti-LBP (IP:11836-1-AP, 4ug; Detection:11836-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 11836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 11836-1-AP (LBP Antibody) at dilution of 1:50.

• 

  IF Staining of L02 using 11836-1-AP

  Immunofluorescent analysis of L02 cells using 11836-1-AP (LBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11836-1-AP

  Immunofluorescent analysis of HepG2 cells, using LBP antibody 11836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 11836-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LBP antibody (11836-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules A431, cellules A549, cellules HeLa, cellules Jurkat, cellules L02, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules L02, cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

11836-1-AP cible LBP dans les applications de WB, IP, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LBP Protéine recombinante Ag2431
• Nom complet: lipopolysaccharide binding protein
• Masse moléculaire calculée: 481 aa, 53 kDa
• Poids moléculaire observé: 63-67 kDa
• Numéro d’acquisition GenBank: BC022256
• Symbole du gène: LBP
• Identification du gène (NCBI): 3929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).

Publications

Species	Application	Title
		J Neurosurg; Association of decreased levels of lipopolysaccharide-binding protein with OKN-007-induced regression of tumor growth in an F98 rat glioma model.; Authors - Nataliya Smith
human	WB	Cancer Sci; Circulating exosomes contain protein biomarkers of metastatic non-small-cell lung cancer.; Authors - Ning Wang
rat	IHC	J Biomed Mater Res A; The characteristic regulation of gene expression Lbp and Sod3 in peri-implant connective tissue of rats.; Authors - Takafumi Kobayashi