Anticorps Recombinant de lapin anti-LC3B

• Phare
• Validé par KD/KO

LC3B Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5P12

N° de cat : 81004-1-PBS

Synonymes

LC3, MAP1LC3B, 5P12, ATG8F, Autophagy-related ubiquitin-like modifier LC3 B

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Galerie de données de validation

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  WB analysis using 81004-1-RR (same clone as 81004-1-PBS)

  Untreated and chloroquine treated HEK-293 cells, untreated and chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

  WB result of LC3B antibody (81004-1-RR; 1:20000; room temperature for 1.5 hours) with negative Control and LC3 knockout HeLa cells. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81004-1-RR (same clone as 81004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 81004-1-RR (same clone as 81004-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81004-1-RR (LC3B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81004-1-RR (same clone as 81004-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3B antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 81004-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81004-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81004-1-RR against Human LC3 were performed. The affinity constant is 0.967 nM.

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Informations sur le produit

81004-1-PBS cible LC3B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LC3B Protéine recombinante Ag6144
• Nom complet: microtubule-associated protein 1 light chain 3 beta
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14-18 kDa
• Numéro d’acquisition GenBank: BC067797
• Symbole du gène: LC3B
• Identification du gène (NCBI): 81631
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B.

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