Anticorps Polyclonal de lapin anti-LC8/DYNLL1

LC8/DYNLL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18130-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 18130-1-AP (same clone as 18130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

  IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18130-1-AP (same clone as 18130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18130-1-AP (same clone as 18130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 18130-1-AP (same clone as 18130-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18130-1-PBS cible LC8/DYNLL1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LC8/DYNLL1 Protéine recombinante Ag12710
• Nom complet: dynein, light chain, LC8-type 1
• Masse moléculaire calculée: 89 aa, 10 kDa
• Poids moléculaire observé: 8-10 kDa
• Numéro d’acquisition GenBank: BC100289
• Symbole du gène: DYNLL1
• Identification du gène (NCBI): 8655
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LC8 (also known as DYNLL1) is a homodimeric sequence-specific chaperone that promotes the ordered (typically homo-) oligomerization of more than a hundred protein targets. LC8/DYNLL1 has previously been proposed to function as an inhibitor of the NF-κB pathway, based on findings that it can bind directly to the IκBα regulatory region(PMID: 18579519). LC8/DYNLL1 regulates apoptotic activities of BCL2L11 by sequestering it to microtubules.

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