Anticorps Polyclonal de lapin anti-LCE1B

LCE1B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21771-1-PBS

Synonymes

LEP2, SPRL2A, Small proline-rich-like epidermal differentiation complex protein 2A, Late envelope protein 2, late cornified envelope 1B

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Galerie de données de validation

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  WB analysis of A375 using 21771-1-AP (same clone as 21771-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 21771-1-AP (same clone as 21771-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skin using 21771-1-AP (same clone as 21771-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 21771-1-AP (same clone as 21771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 21771-1-AP (same clone as 21771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus using 21771-1-AP (same clone as 21771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus using 21771-1-AP (same clone as 21771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A375 using 21771-1-AP (same clone as 21771-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using LCE1B antibody (21771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21771-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21771-1-PBS cible LCE1B dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LCE1B Protéine recombinante Ag16442
• Nom complet: late cornified envelope 1B
• Masse moléculaire calculée: 118 aa, 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC104232
• Symbole du gène: LCE1B
• Identification du gène (NCBI): 353132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).

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