Anticorps Recombinant de lapin anti-LDHA-Specific

• Phare
• Validé par KD/KO

LDHA-Specific Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240885F4

N° de cat : 84198-1-PBS

Synonymes

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

---

Galerie de données de validation

• 

  WB analysis of MDA-MB-231 using 84198-1-RR (same clone as 84198-1-PBS)

  WB result of LDHA-Specific antibody (84198-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84198-1-RR (same clone as 84198-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-RR, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84198-1-PBS

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-PBS, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84198-1-PBS cible LDHA-Specific dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lactate dehydrogenase A
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: NM_005566
• Symbole du gène: LDHA
• Identification du gène (NCBI): 3939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.

{{ptg:RelatedPrimaryAntibodies}}