Anticorps Recombinant de lapin anti-LDHA-Specific

• Phare
• Validé par KD/KO

LDHA-Specific Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240885C7

N° de cat : 84198-3-RR

Synonymes

LDHA, 240885C7, Cell proliferation-inducing gene 19 protein, EC:1.1.1.27, lactate dehydrogenase A

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Galerie de données de validation

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  WB analysis of MDA-MB-231 using 84198-3-RR

  WB result of LDHA-Specific antibody (84198-3-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells.

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  WB analysis using 84198-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84198-3-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 84198-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-3-RR, Clone:240885C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MDA-MB-231, cellules NIH/3T3, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84198-3-RR cible LDHA-Specific dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lactate dehydrogenase A
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: NM_005566
• Symbole du gène: LDHA
• Identification du gène (NCBI): 3939
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.

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