Anticorps Monoclonal anti-LDHB

LDHB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5E9G1

N° de cat : 66425-1-PBS

Synonymes

lactate dehydrogenase B, 5E9G1, EC:1.1.1.27, LDH, LDH B

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Galerie de données de validation

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  WB analysis using 66425-1-Ig (same clone as 66425-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66425-1-Ig (LDHB antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66425-1-Ig (LDHB antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66425-1-Ig (same clone as 66425-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66425-1-Ig (LDHB antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66425-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66425-1-PBS cible LDHB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: LDHB Protéine recombinante Ag6605
• Nom complet: lactate dehydrogenase B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002362
• Symbole du gène: LDHB
• Identification du gène (NCBI): 3945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.

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