Anticorps Recombinant de lapin anti-LDHB

LDHB Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1M20

N° de cat : 81963-1-PBS

Synonymes

lactate dehydrogenase B, 1M20, EC:1.1.1.27, LDH, LDH B

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Galerie de données de validation

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  WB analysis using 81963-1-RR (same clone as 81963-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81963-1-RR (LDHB antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

  IP result of anti-LDHB (IP:81963-1-RR, 4ug; Detection:81963-1-RR 1:50000) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDHB antibody (81963-1-RR, Clone: 1M20 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81963-1-RR (same clone as 81963-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LDHB (81963-1-RR, Clone:1M20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81963-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81963-1-PBS cible LDHB dans les applications de WB, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: LDHB Protéine recombinante Ag6605
• Nom complet: lactate dehydrogenase B
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002362
• Symbole du gène: LDHB
• Identification du gène (NCBI): 3945
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of pyruvate and NADH to lactate and NAD+ in the glycolytic pathway. LDH comprises LDHA and LDHB, two subunits that are encoded by independent genes. In muscle or liver, most of the LDH is composed of four LDHA subunits, and preferably catalyzes the reduction of pyruvate to lactate. In heart and brain, LDH is mainly composed of four LDHB subunits, and predominantly catalyzes the oxidation of lactate to pyruvate. In other tissues, LDH is composed of both LDHA and LDHB. (PMID: 26269128, 31804482)

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