Anticorps Polyclonal de lapin anti-LDHC-Specific

LDHC-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19989-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human testis using 19989-1-AP (same clone as 19989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  WB analysis using 19989-1-AP (same clone as 19989-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 19989-1-AP (same clone as 19989-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 19989-1-AP (same clone as 19989-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 19989-1-AP (same clone as 19989-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19989-1-AP (LDHC-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  IP result of anti-LDHC-Specific (IP:19989-1-AP, 4ug; Detection:19989-1-AP 1:500) with mouse testis tissue lysate 2400ug. This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19989-1-AP (same clone as 19989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 19989-1-AP (LDHC-Specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19989-1-AP (same clone as 19989-1-PBS)

  Immunofluorescent analysis of Hela cells, using LDHC antibody 19989-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19989-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19989-1-PBS cible LDHC-Specific dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lactate dehydrogenase C
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 30-36 kDa
• Numéro d’acquisition GenBank: NM_002301
• Symbole du gène: LDHC
• Identification du gène (NCBI): 3948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

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