Anticorps Polyclonal de lapin anti-LDHD

LDHD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14398-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14398-1-AP (same clone as 14398-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14398-1-PBS cible LDHD dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LDHD Protéine recombinante Ag5897
• Nom complet: lactate dehydrogenase D
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 45-54 kDa
• Numéro d’acquisition GenBank: BC047902
• Symbole du gène: LDHD
• Identification du gène (NCBI): 197257
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

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