Anticorps Polyclonal de lapin anti-LDLR

• Phare
• Validé par KD/KO

LDLR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

88

N° de cat : 10785-1-AP

Synonymes

FH, FHC, LDL receptor, LDLR

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Galerie de données de validation

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  WB analysis of HeLa using 10785-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10785-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10785-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10785-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10785-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10785-1-AP

  IP Result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of mouse brain using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human brain using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human pancreas using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10785-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10785-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10785-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10785-1-AP

  1X10^6 HeLa cells were stained with .2ug LDLR antibody (10785-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HL-60, cellules Jurkat, cellules Raji, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de cancer du côlon humain, tissu hépatique de souris, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 81 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

10785-1-AP cible LDLR dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LDLR Protéine recombinante Ag1236
• Nom complet: low density lipoprotein receptor
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 100-160 kDa
• Numéro d’acquisition GenBank: BC014514
• Symbole du gène: LDLR
• Identification du gène (NCBI): 3949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
human	WB	Cell Metab; Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.; Authors - Jiang Wang
human,mouse	WB,IF	Sci Transl Med; Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology; Authors - Su'an Tang
mouse	WB,IP	J Clin Invest; Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.; Authors - Shanshan Zhong ; KO Validated
human	WB	Nat Commun; A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.; Authors - Yunkai Zhu
mouse	IF	Adv Healthc Mater; Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer.; Authors - Yi Pan

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